Not available outside of the UK & Ireland.

Application

Ascorbate oxidase, from Cucurbita sp., may be used to study oxidative stress and heat stress response and tolerance. Ascorbate oxidase, from Sigma, has been used in ascorbic acid assays to study the heat stress response of Arabidopsis .

Ascorbate Oxidase from Cucurbita sp. has been used: as a supplement in the culture medium for differentiation into osteoblasts to oxidize ascorbic acid producing monodehydroascorbate in monodehydroascorbate reductase assay in determining ascorbate (AsA) and dehydroascorbate (DHA) concentrations

Biochem/physiol Actions

Ascorbate oxidase converts ascorbic acid to dehydroascorbic acid. It is highly specific for L-ascorbic acid and a few analogs. Ascorbate oxidase exists as a dimer and has a molecular weight of approximately 140 kDa.

Ascorbate oxidase (AO) participates in cell growth by regulating reduction/oxidation (redox) of the apoplast. This enzyme can synthesize the oxidative molecule dehydroascorbate acid (DHA) in the apoplast. It is also involved in cell elongation and enlargement development.

General description

Ascorbate oxidase is a homodimeric enzyme, which comprises 552 amino acid residues per subunit (zucchini). It corresponds to a molecular mass of 70kDa per subunit. This enzyme is mainly found in plants, fungi and eubacteria.

Packaging

100, 250, 1000 units in poly bottle

Physical form

Lyophilized powder containing buffers and sucrose as stabilizer.

Unit Definition

One unit will oxidize 1.0 µmole of L-ascorbate to dehydroascorbate per min at pH 5.6 at 25 °C.