Not available outside of the UK & Ireland.

Application

Urease fromCanavalia ensiformis(Jack bean) has been used in purification and crystallization studies.

Urease from Canavalia ensiformis may be used for urea determination of various samples, such as legumes . It may be useful for the detection of pathogens as well as heavy-metal ions.

Biochem/physiol Actions

Urease catalyzes the hydrolysis of urea into carbon dioxide and ammonia. Urease is involved in nitrogen metabolism and urea degradation. Urease from Canavalia ensiformis binds 2 nickel ions per subunit .

General description

Subunit molecular weight: ~90,770 Composed of six subunits with total molecular weight: ~544,620

Urease(Ure) enzyme is present in the cytosol. It is active at acidic pH and is expressed at 28°C. Ureaseconsists of nickel and is present in archaea, bacteria, unicellular eukaryotes and plants.

Other Notes

Application in (selective) hydrolysis/condensations; structure.

Unit Definition

1 U corresponds to the amount of enzyme which hydrolyzes 1 µmol urea (Cat. No. 51459) per minute at pH 8.0 and 25 °C.