Not available outside of the UK & Ireland.

Application

MeO-SucAAPVpNA has been used to measure neutrophil elastase activity:in response to zinc supplementation using human and mice neutrophils in vitroto determine the functional properties of aged neutrophils to assess their role in supporting metastasisto determine the anti-elastase action of Sclerocarya birrea (A. Rich.) Hochst

Biochem/physiol Actions

Human leukocyte elastase (HLE) is a serine proteinase associated with inflammation and tissue degradation. It is one of the most destructive proteases and its role in the development of pulmonary emphysema has been investigated. This peptide is selectively hydrolyzed by HLE, and other elastase of different species and cells. Cleavage of MeO-SucAPVVpNA by an elastase-like protease is critical for the synthesis of platelet-activating factor (PAF) induced by tumor necrosis factor-α (TNF-α) and interleukin-1α (IL-1α). The rate of enzymatic hydrolysis of the substrate is followed by the increase in absorbance due to the release of 4-nitroaniline. The absorbance is routinely measured at 400 nm or higher.

Packaging

10, 50, 250 mg in poly bottle

1 g in poly bottle

Substrates

Substrate for human leukocyte elastase.