Not available outside of the UK & Ireland.

Application

BCIP^®/NBT-Blue Liquid Substrate System for Membranes has been used in colorimetric detection of alkaline phosphatase (ALP) activity such as western blot analysis.

Caution

Stable at least one year at 2-8 °C. This substrate is light-sensitive.

General description

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-bromo4-chloro-3-indolyl phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end-product that is blue to purple and can be observed visually. BCIP^®/NBT-blue liquid substrate system for membranes is a specifically formulated liquid enzyme substrate that is stable and optimized for blotting applications offers maximum convenience, time savings, and consistency.

Legal Information

BCIP is a registered trademark of Sigma-Aldrich Co. LLC

Other Notes

Not recommended for microwell (e.g., ELISA) or immunohistochemical applications.