Not available outside of the UK & Ireland.

Application

Nα-Tosyl-L-lysine chloromethyl ketone hydrochloride has been used:in chymotrypsin purification to prevent binding of trypsins to the affinity support by inhibiting them in the crude extractas a protease inhibitor in tissue and cell homogenizationas a trypsin inhibitor to determine the specific protease activity levels

Biochem/physiol Actions

Nα-Tosyl-L-lysine chloromethyl ketone hydrochloride (TLCK) blocks the lipopolysaccharide (LPS)- or cytokine-induced activation of nuclear factor κB (NF-κB), which, in turn, blocks the induction of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) transcription. Blocks activation of pp70s6k by all mitogens. Blocks apoptosis in cell lines by inhibiting the processing of caspases in some cell lines and to some stimuli.

Caution

Stable at least two years when stored desiccated at −20°C.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.