Not available outside of the UK & Ireland.

Application

Research CategoryNeuroscience

Used to detect/quantify: BDNF

This ChemiKine Brain Derived Neurotrophic Factor, Sandwich ELISA is used to measure & quantify BDNF levels in Neuroscience research.

Components

ChemiKine BDNF ELISA Plate: (Part No. 60238) Two Pre-coated with Rabbit anti-Human BDNF Polyclonal Antibody 96-Well Immunoplates sealed in foil pouches.Wash Buffer Concentrate: (Part No. 60245) One 100 mL (10X) bottle of Concentrate.Standard/Sample Diluent: (Part No. 60240) One 60 mL bottle (Ready to Use).BDNF Standard (Recombinant Human): (Part No. 60237) Two vials (Lyophilized).Biotinylated Mouse anti-Human BDNF Monoclonal Antibody: (Part No. 60583) One 25 μL vial.Streptavidin-Enzyme conjugate: (Part No. 60582) One 50 mL vial of HRP conjugated Streptavidin.TMB/E Solution: (Part No. 60096) Two 10 mL bottles of a Ready to Use solution of 3,3′,5,5′-tetramethylbenzidine in a proprietary buffer with enhancer.Stop Solution (Part No. 60260): One 22 mL bottle of an HCl solution.

Disclaimer

For research use only. Not for use in diagnostic procedures.

General description

ChemiKine™ Brain Derived Neurotrophic Factor (BDNF) kit is a sandwich enzyme immunoassay (EIA), which measures BDNF. The kit will measure BDNF from human and rat.

Test Principle:

With the ChemiKine BDNF assay system, rabbit polyclonal antibodies generated against human BDNF are coated onto a microplate and are used to capture BDNF from a sample. BDNF specific, biotin conjugated, mouse monoclonal antibodies detect the captured BDNF. After addition of streptavidin-enzyme, substrate and stop solution the amount of BDNF is determined. The standard curve demonstrates a direct relationship between Optical Density (OD) and BDNF concentration: i.e., the higher the OD the higher the BDNF concentration in the sample.

Application:

ChemiKine BDNF kit is designed to measure the amount of BDNF in cell culture supernatants, tissue homogenates and biological fluid (serum, plasma, and serum-free) samples from human and rat. There are enough reagents included in this kit for two 96-well immuno-assay plates. Running duplicate wells for samples and standards is recommended.

For Research Use Only. Not for use in diagnostic procedures.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage and Stability

Precautions:

⋅ Wash Buffer and Sample Buffer contain thimerosal. Thimerosal is highly toxic by inhalation, contact with skin or if swallowed. Thimerosal is a possible mutagen and should be handled accordingly.

⋅ The instructions provided have been designed to optimize the kit′s performance. Deviation from the instructions may result in suboptimal performance of the kit and the failure to produce accurate data.