cgtmag-98k-03

Not available outside of the UK & Ireland.

Application

Analytes: Amylin* (Total), Ghrelin* (Active), GIP (Total), GLP-1* (Active), Glucagon, Insulin, Leptin, Pancreatic Polypeptide (PP) and PYY (total) Recommended Sample Type: Canine serum, plasma, tissue supernatants or tissue extraction samples; (*) specific analytes require protease inhibitor(s) during sample collection Recommended Sample Dilution: 50 µL per well of undiluted serum or plasma Assay Run Time: Overnight (16-18 hr) at 2-8°C Research Category: Metabolism Research Subcategory: Obesity, Diabetes

Components

Canine Gut Hormone Standard, 1 vial, lyophilizedCanine Gut Hormone Control 1, 1 vial, lyophilizedCanine Gut Hormone Control 2, 1 vial, lyophilizedAssay Buffer, 1 bottle, 30 mLBead Diluent, 1 bottle, 1 mLSerum Matrix, 1 bottle, lyophilizedWash Buffer, 10X (0.05% Proclin), 2 bottlesl, 30 mLCanine Gut Hormone Detection Antibodies, 1 bottle, 5.5 mLStreptavidin-Phycoerythrin, 1 bottle, 5.5 mLMixing Bottle, 1 bottleSample Plate, 96 well format, 1 plate2 Foil Plate Sealers

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

General description

Gut hormones are a group of polypeptides secreted from the gastrointestinal tract. These peptide hormones play very important roles in the regulation of gut motility and secretion, pancreatic islet hormone secretion, appetite, and energy expenditure. The MILLIPLEX^® Canine Gut Hormone Panel is a 9-plex kit to be used for the simultaneous quantification of any or all of the following analytes in canine plasma, serum or tissue supernatants or tissue extraction samples: Amylin (total), Active Ghrelin, GIP, GLP-1, Glucagon, Insulin, Leptin, PP and PYY. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate. The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume. Panel Type: Metabolism

Legal Information

xMAP is a registered trademark of Luminex Corp

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

Everything you need in a single kit.

Specificity

Cross ReactivtyNo significant cross-reactivity with other hormones tested.

Storage and Stability

Recommended storage for kit components is 2 - 8°C

assay range	sensitivity: 0.6 pg/mL(Minimum Detectable Concentration (MinDC +2SD); GIP (total)), sensitivity: 3.3 pg/mL(Minimum Detectable Concentration (MinDC +2SD); Ghrelin (active)), sensitivity: 3.6 pg/mL(Minimum Detectable Concentration (MinDC +2SD); PP), sensitivity: 9.0 pg/mL(Minimum Detectable Concentration (MinDC +2SD); GLP-1 (active)), accuracy: 98%(PYY(total)), standard curve range: 0.5-2,000 pg/mL(GIP), sensitivity: 92.1 pg/mL(Minimum Detectable Concentration (MinDC +2SD); Leptin), accuracy: 99%(Ghrelin(Active)), sensitivity: 43.6 pg/mL(Minimum Detectable Concentration (MinDC +2SD); Glucagon), sensitivity: 13.1 pg/mL(Minimum Detectable Concentration (MinDC +2SD); PYY (Total)), standard curve range: 24.4-100,000 pg/mL(Insulin, Leptin), standard curve range: 0.7-3,000 pg/mL(Ghrelin (Active)), accuracy: 99%(Leptin), standard curve range: 4.9-20,000 pg/mL(Amylin (Total), Glucagon), sensitivity: 5.9 pg/mL(Minimum Detectable Concentration (MinDC +2SD); Amylin (total)), accuracy: 100%(GLP-1(Active)), standard curve range: 2.4-10,000 pg/mL(GLP-1 (Active), PP, PYY (Total)), accuracy: 99%(PP), accuracy: 99%(GIP), sensitivity: 79.3 pg/mL(Minimum Detectable Concentration (MinDC +2SD); Insulin), accuracy: 103%(Amylin(Total)), accuracy: 101%(Glucagon)
detection method	fluorometric (Luminex xMAP)
manufacturer/tradename	Milliplex^®
Quality Level	200
shipped in	wet ice
species reactivity	canine
technique(s)	multiplexing: suitable