BTX AgilePulse In Vivo IM (Intra-Muscular) Generator Only

Code: 470501n D2-0

Advanced electroporation solution providing maximum efficiency DNA and RNA delivery to skin, muscle, and tumor tissues. The AgilePulse In Vivo System, used for animal immunisation, vaccine developm...


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£23,084.00 EACH
£27,700.80 inc. VAT

Advanced electroporation solution providing maximum efficiency DNA and RNA delivery to skin, muscle, and tumor tissues. The AgilePulse In Vivo System, used for animal immunisation, vaccine development, and gene therapy, provides an intra-muscular, intra-dermal, or intra-tumor electroporation solution to produce maximum transfection efficiency. Traditional in vivo nucleic acid delivery systems, such as gene gun delivery, suffer from poor efficiency. The AgilePulse In Vivo System effectively introduces DNA or RNA into the target tissue, representing a powerful and safe means for stimulating an immune response that recognises and eliminates target molecules in the body.

For vaccine applications, DNA vaccination through the dermal layer is preferred since it is an easily accessible site that is immunologically active. After direct injection of plasmid DNA in the dermal layer, a programmed sequence of electric pulses is applied through a miniature parallel needle electrode array to promote cellular uptake and transfection. Cells in the surrounding tissue are transfected, including dendritic antigen-presenting cells and mesenchymal origin cells. Gene expression simulates the immune system to respond to the secreted antigen. Gene expression in skin is 100-fold higher when delivery is enhanced by electroporation compared to simply injecting plasmid DNA (Roos, et. al. 2009).

  • Simple user interface: touch screen user interface, USB key data storage, and Windows® Mobile 6.0 operating system. The system automatically stores a digitized record of all pulse parameters for quality control. EN 60601-1, EN 60601-1-1 EN 60601-1-2, UL60601-1 and CSA601.1 compliant.
  • Pulse Agile® Advantage: patented Pulse Agile technology combines a unique sequence of short, high-intensity pulses to open pores in cell membranes, followed by long, low-intensity pulses to further drive the DNA into cells via electrophoresis. The adjustable pulses improve efficiency while maintaining cell viability. The specialised use of the Pulse Agile waveform has shown to significantly enhance antigen specific CD8+ T-cell response when standard protocols do not.
  • Uniform, reliable electroporation: AgilePulse parallel-needle arrays produce uniform electric fields across the treatment area for a more thorough transfection of the tissue. Electrode impedance is monitored through software analysis to ensure reliable electrode placement in tissue each and every time.
  • Simple, fast treatment: simple yet effective intra-dermal or intramuscular electrode design allows for shorter delivery times of less than a second. The electrodes provide a uniform pulse to cover and target larger areas of tissue using a single pulsing application.
  • Optimal design: miniature, 2-mm length needles easily penetrate the layers of skin or muscle and target cells for high efficiency gene delivery.
  • Safety: each needle electrode array comes with a safety cover and easy grip sides to make the electrode insertion procedure simple and safe.

Contents:

  • 47400N: AgilePulse In Vivo ID (Intra-Dermal) generator, handle for parallel needle array, 3 x 4-needle parallel needle array, 3 x 6-needle parallel needle array, ID software
  • 470401N: AgilePulse In Vivo ID (Intra-Dermal) generator only
  • 470500N: AgilePulse In Vivo IM (Intra-Muscular) generator, handle for parallel needle array, 3 x 4-needle parallel needle array, 3 x 6-needle parallel needle array, IM software
  • 470501N: AgilePulse In Vivo IM (Intra-Muscular) generator only

AgilePulse ID and IM Generator Specifications
User InterfaceTouch screen display, footswitch
Voltage Range50 to 1000V
Pulse Width Range0.050 to 10ms
Pulse Interval0.200 to 1000ms (5kHz to 1Hz)
Data ExportUSB flash drive
Dimensions (W x H x D) (with handle)32 x 20 x 40cm
Weight11.3kg
Operating Temperature10 to 40°C
Mains Voltage100 to 250V AC
Fuse5Amp slo-blo, 5 x 20mm
SoftwareIM (Intra-Muscular) or ID (Intra-Dermal)
AgilePulse Waveform Generator Selection Guide
Tissue TypeTissue Load RangeRecommended Generator
Skin, Intradermal1100 to 2000 OhmsAgilePulse ID
Tibialis muscle800 to 1500 OhmsAgilePulse ID
Quadriceps muscle, small animal200 to 400 OhmsAgilePulse IM
Quadriceps muscle, large animal80 to 250 Ohms AgilePulse IM
Code
Description
Unit Size
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470400N
Unit:EACH
List Price: £25,479.00
Source:List Price
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470401N
Unit:EACH
List Price: £23,084.00
Source:List Price
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470500N
Unit:EACH
List Price: £25,479.00
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Code
Description
Unit Size
List Price
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470040
Unit:EACH
List Price: £389.00
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470043
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List Price: £524.00
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470045
Unit:EACH
List Price: £524.00
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470050
Unit:EACH
List Price: £389.00
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470060
Unit:EACH
List Price: £389.00
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470070
Unit:EACH
List Price: £389.00
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470080
Unit:EACH
List Price: £389.00
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470086
Unit:EACH
List Price: £524.00
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470092
Unit:EACH
List Price: £1,476.00
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