The BTX pulse switcher is a plug-in accessory and accompanying firmware package which upgrades the square wave pulsing capabilities of 7-inch touchscreen ECM 830 and Gemini X2 waveform generators. A new protocol category type, pulse switcher protocols, is available. Pulse switcher protocols allow a user to create and run protocols containing groups of square wave pulses with different polarity, pulse number, pulse length, and pulse interval characteristics.

• Pulse switcher user defined protocols may be programmed with up to 10 groups of square wave pulses with separately defined polarity (+ or -) for each group. The user may thus incorporate bipolar switching into their electroporation protocols
• The maximum number of pulses per group in a pulse switcher user defined protocol is 100 pulses
• The number of pulses, pulse length, and pulse interval is separately defined for each group of pulses in pulse switcher user defined protocols
• The minimum interval between pulses and between groups of pulses for pulse switcher user defined protocols is 50ms
• The maximum number of pulses per pulse switcher user defined protocol is 1000 pulses
• 11 new pulse switcher preset electroporation protocols have been added, utilising bipolar switching capabilities for in vivo electroporation applications

Square wave user defined protocol features on the ECM 830 or Gemini X2 are enhanced with the installation of the pulse switcher:

• The maximum number of pulses per square wave user defined protocol is increased to 100 pulses
• The minimum interval between pulses for square wave user defined protocols is decreased to 50ms
• Exponential decay wave pulsing features on the Gemini X2 are unaffected by the BTX pulse switcher

Features and benefits:

• Significantly expanded pulsing application capabilities - the ECM 830 and Gemini X2 solutions already have an established electroporation publication record, including use in CRISPR gene editing technologies. Adding a BTX Pulse Switcher to your system further expands your application protocol capabilities.
• Ideal for high efficiency transfection of mammalian cells and tissues - including difficult to transfect cell types, such as stem cells and primary cells.
• Simplify execution of advanced protocols, including automatic, hands-free bipolar switching.
• Suitable for many applications, including in vitro, in vivo, in utero, in ovo, and ex vivo.