Tris-EDTA buffer solution, 100 x, for molecular biology

Code: t9285-100ml D2-231

Not available outside of the UK & Ireland.

Application

Suitable for storage and isolation of RNA and DNA, including purified plasmid DNA stocks.Tris-EDTA buffer solution has been used in culture medium.Tris-EDTA buffe...


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$33.79 100ML
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Not available outside of the UK & Ireland.

Application

Suitable for storage and isolation of RNA and DNA, including purified plasmid DNA stocks.Tris-EDTA buffer solution has been used in culture medium.Tris-EDTA buffer solution has been used for the preparation of AmpC disks for permeabilization of Tris-EDTA to release β-lactamases from bacterial cell.It has been used for the suspension of bacterial spores.It has also been used as a buffer in immunohistochemistry.

Components

TE Buffer contains 1M Tris-HCl (pH approximately 8.0), containing 0.1M EDTA.

General description

TE buffer is the most commonly used buffer solution in molecular biology. "TE" is a component of Tris (a common pH buffer) and EDTA (molecule that chelate cations). TE (Tris EDTA) Buffer solubilizes DNA and RNA while protecting it from degradation. TE Buffer is used in nucleic acid isolation, which may be done prior to Northern or Southern blot hybridization.

foreign activityDNase, RNase, none detected
formliquid
gradefor molecular biology
InChI keyVLEIUWBSEKKKFX-UHFFFAOYSA-N
InChI1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2
pH7.8-8.2 (1 × in water)
Quality Level200
SMILES stringNC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
sterility0.2 µm filtered
storage temp.room temp
This product has met the following criteria: