Not available outside of the UK & Ireland.

Application

SigmaMarkers are designed for use with Laemmli SDS-PAGE, the PhastSystem (Pharmacia) and Schagger and von Jagow SDS-PAGE (Tricine). SigmaMarkers cover the range of molecular weights common to most proteins or their subunits. The wide, high and low range markers are lyophilized with sample buffer so that they are ready-to-use after reconstitution with deionized water. The markers are formulated to yield a distribution of well-defined bands of approximately equal intensity after electrophoresis and Coomassie blue staining.

SigmaMarker has been used as a protein marker in sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE).

Legal Information

SigmaMarker is a trademark of Sigma-Aldrich Co. LLC

Other Notes

Protein (mol. wt.)Aprotinin, bovine lung (6,500)A-Lactalbumin, bovine milk (14,200)Trypsin inhibitor, soybean (20,000)Trypsinogen, bovine pancreas (24,000)Carbonic anhydrase, bovine erythrocytes (29,000)Glyceraldehyde-3-phosphate dehydrogenase, rabbit muscle (36,000)Ovalbumin, chicken egg (45,000)Glutamic dehydrogenase, bovine liver (55,000)Albumin, bovine serum (66,000)Phosphorylase B, rabbit muscle (97,000)β-Galactosidase, E. coli (116,000)Myosin, porcine heart (200,000)

Will yield 12 protein bands with Coomassie Blue staining.

Reconstitution

Reconstitution with 100 µl of deionized water results in a solution containing 2-3.5 mg of protein per ml of 62 mM Tris-HCl, pH 6.8, 1 mM EDTA, 4% sucrose, 10 mM TCEP, 2% SDS, and 0.01% bromophenol blue.