5X Protein Loading Buffer is a reducing sample buffer for SDS-polyacrylamide gel electrophoresis (SDS-PAGE). 5X Protein Loading Buffer contains 1.0M Tris HCl (pH 8.5), 8% (w/v) lithium dodecyl sulphate, 40% (v/w) glycerol, 2mM EDTA, 0.5M DTT and tracking dye in distilled/deionized water.
More concentrated loading buffer means less sample dilution, with more protein loaded per well. The use of lithium dodecyl sulphate, in place of the more common sodium salt, give the best possible resolution.
Keep frozen until ready to use. Protect from physical damage. Isolate from incompatible materials. Avoid contact and inhalation. Do not get in eyes, on skin, on clothing. Wash thoroughly after handling.
- Load more sample per well
- Lithium Dodecyl Sulphate give improved resolution
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