Tris-Glycine Electro blotting Buffer (10X) is a concentrated solution in distilled/deionized water. This buffer is intended to act as the tank buffer in Western electro blotting procedures. It is also the recommended buffer for use with nitrocellulose and nylon membranes.
Tris-Glycine Electro blotting Buffer (10X) is already in solution which eliminates the weighing, mixing and adjusting of the pH necessary with powdered buffers. To prepare 1 litre of working strength buffer, add 100 ml of Tris-Glycine Electro blotting Buffer to 200 ml of methanol and 700 ml of distilled/deionized water.
Tris-Glycine Electro blotting Buffer (10X) contains 0.25M Tris base and 1.92M glycine, with a pH of 8.4
Keep in a tightly closed container, stored in a cooled, dry, ventilated area. Avoid contact and inhalation. Do not get in eyes, on skin, on clothing. Wash thoroughly after handling.
- Manufactured with ultra-pure electrophoresis grade reagents
- 18 MegOhm water & 0.2 Micron filtration
- Saves time and improves reproducibility in Western Blotting
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