The AxyPrep MAG PCR normaliser kit utilises a paramagnetic beads-based purification system for post PCR clean-up. AxyPrep MAG PCR normaliser beads have limited binding surface, therefore by limiting the amount of beads added in a given purification reaction, pre-defined amount of DNA can be isolated based on customers need. PCR normalisation is accomplished during this purification process so that additional DNA quantification and dilution are not necessary. Time, labour, and reagent cost are greatly saved with the AxyPrep MAG unique normalisation purification chemistry, therefore efficiently streamlining genomics downstream applications. The protocol mainly consists of binding, washing, and elution steps, and it can be performed directly in the thermal cycling plate and requires no centrifugation or filtration. The process can be automated with walk away solution for high throughput applications.
- Dual action: Normalisation and post PCR clean-up enables significant reduction in processing and faster turnaround times
- Scalable: Tube, 96 and 384 well plate formats
- Manual and automation-compatible protocol
- Compatible with several downstream applications
- No centrifugation or filtration required
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