Not available outside of the UK & Ireland.

Components

pT7-FLAG-2 Expression Vector, 10 µg (P6867) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA. pT7-FLAG-2-BAP Control Vector 1 µg ( P7117) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

General description

pT7-FLAG-2 is a 4.8 kb E . coli expression vector used for cloning and cytoplasmic expression of a properly inserted open reading frame as a C-terminal FLAG^® fusion protein containing the FLAG epitope (DYKDDDDK). The promoter region of the very strong phage T7 promoter drives transcription of ORFFLAG fusion constructs. This vector requires the use of E. coli cells containing a source of the T7 polymerase, such as BL21(DE3)T1R cells, Catalog Number B2935.The C-terminal FLAG fusion protein may be detected using Monoclonal ANTI-FLAG?M2, Catalog NumberF3165, and purified using the ANTI-FLAG M2 Affinity Gel, Catalog Number A2220.Vector Maps and Sequences

Legal Information

pT7-FLAG is a trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Sigma-Aldrich Co. LLC