Not available outside of the UK & Ireland.

Analysis Note

Iron content by ICP reported on a lot-specific basis.

Purity by agarose gel electrophoresis.

Application

Transferrin is the primary iron binding and cell delivery molecule present in serum. Serum-free cell culture systems require a delivery format for iron. Transferrin is the preferential delivery form of iron because cells process transferrin bound iron in a physiologically appropriate way through transferrin receptors on the cell surface. Human holo-transferrin is a high affinity transferrin that can be used with a wide range of cells types. Human holo-transferrin is loaded with iron prior to use and can be added directly to iron poor or iron-free cell culture media.

holo-Transferrin human has been supplemented in Roswell Park Memorial Institute (RPMI) for proerythroblasts proliferation assay and also in culture media for inductively coupled plasma emission spectroscopy.

Biochem/physiol Actions

Provides iron to cells in a physiologically stable and safe form.

Packaging

1 g in poly bottle

50, 100, 500 mg in poly bottle

Preparation Note

Heat treated at 60 °C (minimum) for a minimum of 10 hours.