Not available outside of the UK & Ireland.

Application

ITS Liquid Media Supplement (100×) has been used as a media supplement to culture:α mouse liver 12 (AML-12) cellsrat fetal testis explantshuman dental pulp stem cells (DPSC)

Biochem/physiol Actions

Insulin: a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe an observed mitogenic effect to this property.Transferrin: an iron-transport protein.Selenium: an essential trace element normally provided by serum.

Components

Contains 1.0 mg/ml recombinant human insulin, 0.55 mg/ml human transferrin (substantially iron-free), and 0.5 µg/ml sodium selenite at the 100x concentration.

Other Notes

ITS is a mixture of recombinant human insulin, human transferrin, and sodium selenite. It is a general cell supplement designed for use in non-complex media (e.g. MEM, RPMI-1640) and complex media (e.g. Ham?s F-12, DME/F-12, MEM) with sodium pyruvate.

Preparation Note

Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.