CellSplit® is a completely xeno-free, direct replacement to porcine or bovine-derived trypsin. It has been developed so that you can use the same protocol, eliminating any disruption to your current procedure. CellSplit® is suitable for both serum-free and serum-containing cell culture protocols due to the inactivation by dilution. Unlike trypsin, there is no requirement for specific inhibitors, such as foetal bovine serum (FBS). The active ingredient is of plant origin and manufactured to GMP grade. A cysteine protease that has broad specificity, cleaving the peptide bonds of leucine and glycine. Cysteine proteases have been extensively used for cell and tissue disassociation. CellSplit® has been comprehensively tested with a wide range of cell lines including Vero, HEK, C2, C12, L929, MRC5, LLCPK1, MDCK and CHO. It has also been tested with primary canine stem cells.
- Completely xeno-free
- Not derived from bacterial cultures
- Plant-derived enzyme manufactured to GMP
- UK CA and CE marked
- Simple protocol
- Inactivation by dilution, no need to use inactivation reagents such as FBS
This product has met the following criteria:
 | Sustainable Product Manufacturing Award | - Measures were in place during the manufacture of this product to reduce energy consumption.
- Measures were in place during the manufacture of this product to generate less waste.
- Measures were in place during the manufacture of this product to reduce water usage.
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 | Sustainable Distribution & Packaging Award | - Manufactured in the UK or Ireland
- Country of origin has an Environmental Performance Index (EPI) greater than 60
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To find out more about our Sustainability Attributes, click here.