Application

Trypan Blue solution has been used:in cell viability assay to count viable and dead cellsin rescue assay to count viable cellsin trypan blue exclusion counting method to determine proliferation curveas the saline control and in the preparation of lipopolysaccharide (LPS) solution to confirm the success of LPS infusionin trypan blue dye exclusion test to determine gastric epithelial cells (GEC) viabilityto count GEC cells using a hemocytometer

General description

Trypan Blue (TB), an anionic hydrophilic azo dye, crosses only the cell membranes of dead cells, thereby staining dead tissues/cells blue. It is used in quantitative microscopy for a dye exclusion staining technique to differentiate between live and dead mammalian cells.

Trypan blue is a dye used to distinguish between live and dead cells. It is a vital stain that is not absorbed by healthy viable cells, but stains cells with a damaged cell membrane. This way, only dead cells can be counted. The method is sometimes referred to as the dye exclusion method.

Preparation Note

Prepared in 0.81% sodium chloride and 0.06% potassium phosphate, dibasic.