Core Biogenesis FGF-2 TOP Growth Factor - 1mg

FGF-2-TOP® (formerly STAB) is a stabilised growth factor that offers a novel way to grow FGF-2-dependent cell cultures more efficiently, with fewer media changes. FGF2-TOP® retains full biological activity even after twenty days. The stable level of FGF-2 in culture allows for a more homogenous, undifferentiated stem cell culture, while saving researchers valuable time and money because repeated supplementation with FGF-2 and a daily medium change is not required.

Core Biogenesis FGF-2 TOP® is the 155 aa mature domain of FGF-2, tag-free with nine amino acid substitutions to enhance stability without impacting bioactivity developed by Dvorak et al. 2018. This increases the functional half-life of the protein from 10 h (wild-type) to >7 days (FGF2-TOP®) in cell culture conditions at 37°C.

• FGF-2 is produced in plant seeds of Camelina sativa bred in our laboratories in Strasbourg, France
• ≥95% measured by SDS page, and resolved under reduced (R) and non-reduced (NR) conditions
• The biological activity of recombinant FGF-2 STAB® was determined by its ability to promote the proliferation of 3T3 cells cultured in adherent condition. Cell proliferation was measured using non-radioactive colorimetric assay and monitored by absorbance. ED50 is 0.1 ng/mL
• Low endotoxin level ≤0.005ng/µg
• Xeno-free
• Lyophilized, to store at -20°C to -80°C, stable for up to 12 months when stored at -20°C to -80°C. Once it is reconstituted it can be stored at -20ºC or -80ºC for up to 1 year