Application

Iscove′s Modified Dulbecco′s Medium has been used to culture:human hepatocellular carcinoma (HCC) cell line HepG2 as monolayersto culture synovial tissue samplesbacterial strain inoculum for stimulation of immune cell experiments

General description

Iscove′s Modified Dulbecco′s Medium contains selenium, additional amino acids and vitamins, sodium pyruvate, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer, and potassium nitrate instead of ferric nitrate, and is a modification of Dulbecco′s Modified Eagle′s medium (DME). Iscove′s medium can be used to support murine B lymphocytes, bone marrow hemopoietic tissue, B cells stimulated with lipopolysaccharide, T lymphocytes, and various hybrid cells.