The Amersham ECL blocking agent can be used for blocking Amersham Protran nitrocellulose (NC) and Amersham Hybond polyvinylidene fluoride (PVDF) membranes when used along with the ECL chemiluminescence detection reagents. During the Western blotting process, non-specific binding of antibodies to the membrane is detrimental to the specificity and sensitivity of the assay. To overcome this challenge, it is essential to block parts of the membrane not already occupied by proteins. The Amersham ECL blocking agent can be used for blocking Amersham Protran nitrocellulose (NC) and Amersham Hybond polyvinylidene fluoride (PVDF) membranes when used along with the ECL chemiluminescence detection reagents, such as Amersham ECL, ECL start, ECL Prime, and ECL Select. Each pack contains 40g of blocking agent which is sufficient for at least 20 mini blots.

• Application: for blocking nitrocellulose and PVDF membranes to be used with ECL and ECL Plus™ Western Blotting Detection Reagents
• Colour: white
• Components: 40g ECL blocking agent, sufficient for at least 20 mini blots