Alkaline phosphatase dephosphorylates ECF Substrate to produce a fluorescent product, which localizes at the site of catalysis. Product fluoresces at 540 to 560nm when excited at the appropriate wavelength.

• A fluorescent substrate for alkaline phosphatase-based detection of protein blots, for use with fluorescence scanning equipment such as Storm instruments
• For use in western blotting, dot, and slot blotting applications
• Sensitivity comparable to chemiluminescence
• The wider dynamic range of fluorescence scanning systems compared with film allows visualisation and quantitation of weak and dark bands in the same scan
• Eliminates need for darkroom and film
• The following reagents are provided, sufficient for detecting 2500cm² membrane: 36mg ECF substrate and 60mL dilution buffer