Not available outside of the UK & Ireland.

Application

Chloride Assay Kit has been used to measure chloride concentration spectrophotometrically and is used to measure chloride levels in blood plasma of toads Rhinella marina.

General description

Chloride is an essential anion needed for the proper functioning of many critical aspects of metabolism, including aiding in maintenance of the body′s acid-base balance. In Cystic Fibrosis, mutations to the CF transmembrane conductance regulator (CFTCR) gene result in altered sodium and chloride ion transport channels.

Principle

The Chloride Assay kit provides a simple and direct procedure for measuring chloride in a variety of samples, including blood and urine. Chloride concentration is determined by a competition reaction between Hg²⁺ and Fe²⁺ for 2,4,6-Tris(2-pyridyl)-s-triazine(TPTZ). The preferred Hg-TPTZ complex exhibits no color. In the presence of chloride, Hg²⁺ forms HgCl₂, which precipitates, allowing TPTZ to complex with Fe²⁺. The Fe-TPTZ complex results in a colorimetric (620 nm) product proportional to the chloride present.

Suitability

Suitable for the quantification of Chloride in a variety of biological samples. Blood and urine can be used directly after dilution with water