DL-Dithiothreitol, BioXtra, >=99.0% (titration)

Code: d5545-5g D2-231

Not available outside of the UK & Ireland.

Application

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulf...


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£327.00 5G
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Not available outside of the UK & Ireland.

Application

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

Packaging

1, 5, 25 g in glass bottle

absorption≤0.100 at 280 in H2O at 0.1 M, ≤0.400 at 260 in H2O at 0.1 M
anion tracessulfate (SO42-): ≤0.005%
assay≥99.0% (titration)
cation tracesBi: ≤0.0005%, Fe: ≤0.0005%, Cr: ≤0.0005%, Ba: ≤0.0005%, Pb: ≤0.0005%, Al: ≤0.0005%, Na: ≤0.1%, Ca: ≤0.001%, K: ≤0.005%, Cu: ≤0.0005%, Mo: ≤0.0005%, Mg: ≤0.0005%, Cd: ≤0.0005%, Ni: ≤0.0005%, Zn: ≤0.0005%, Mn: ≤0.0005%, Sr: ≤0.0005%, As: ≤0.0001%, Li: ≤0.0005%, Co: ≤0.0005%
formpowder
impuritiesInsoluble matter, passes filter test, ≤0.3% 4,5-dihydroxy-1,2-dithiane
InChI keyVHJLVAABSRFDPM-QWWZWVQMSA-N
InChI1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
mp41-44 °C (lit.)
pH4.0-6.0 (20-25 °C, 0.1 m in H2O)
product lineBioXtra
Quality Level200
reaction suitabilityreagent type: reductant
SMILES stringO[C@H](CS)[C@H](O)CS
solubilityH2O: 0.1 M, clear, colorless
storage temp.2-8°C
Code
Description
Unit Size
List Price
Qty
D5545-25G
DL-Dithiothreitol, BioXtra, >=99.0% (titration)

Unit:25G
List Price: £1,160.00
Source:List Price
ADD
D5545-1G
DL-Dithiothreitol, BioXtra, >=99.0% (titration)

Unit:1G
List Price: £84.30
Source:List Price
ADD
Cas Number3483-12-3
This product has met the following criteria: