CHAPS hydrate, BioXtra, >=98% (TLC)

Code: c5070-5g D2-231

Not available outside of the UK & Ireland.

Application

10mM CHAPS detergent has been used in the process of ion-exchange chromatography for improved purification.0.5 w/v of CHAPS is a component of high salt buffer in ...


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£370.00 5G
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Not available outside of the UK & Ireland.

Application

10mM CHAPS detergent has been used in the process of ion-exchange chromatography for improved purification.0.5 w/v of CHAPS is a component of high salt buffer in protein purification alongside HEPES, NaCl and EDTA.HEPES buffer has been used as lysis buffer in immunoprecipitation, supplemented with 1% CHAPS.

General description

CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent. It is mild and solubilizes membrane proteins and receptors. It is useful for breaking protein-protein interactions. It is a derivative of the naturally occurring bile salts. It has small micellar molecular weight (6,150) and high critical micellar concentration (6 - 10 mM) allowing it to be removed from samples by dialysis.

Packaging

1, 5 g in poly bottle

anion traceschloride (Cl-): ≤0.05%
assay≥98% (TLC)
cation tracesFe: ≤0.0005%, Al: ≤0.0005%, K: ≤0.005%, Cu: ≤0.0005%, Pb: ≤0.001%, Mg: ≤0.0005%, Zn: ≤0.0005%, NH4+: ≤0.05%, Na: ≤0.005%, Ca: ≤0.0005%
descriptionzwitterionic
formpowder
ign. residue≤0.1%
impurities≤0.1% Insoluble matter, ﹤0.04% DMF, ≤0.001% Phosphorus (P)
InChI keySJCUTFKCLFLIFE-JWTJKVBLSA-N
InChI1S/C32H58N2O7S.H2O/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36;/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41);1H2/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-;/m1./s1
mol wt614.88 g/mol (anhydrous basis)
mp157 °C (dec.) (lit.)
product lineBioXtra
Quality Level400
SMILES stringO.C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)C[C@@H]4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C
solubilityH2O: 0.1 M, clear, colorless
technique(s)protein quantification: suitable, protein purification: suitable
Code
Description
Unit Size
List Price
Qty
C5070-1G
CHAPS hydrate, BioXtra, >=98% (TLC)

Unit:1G
List Price: £132.00
Source:List Price
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Cas Number331717-45-4
This product has met the following criteria: