Not available outside of the UK & Ireland.

Application

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Urea has been used in protein resuspension buffer and to denature proteins. It has been used for the isolation of proteins via urea-containing polyacrylamide gels. It has been used to study the conformation of unfolded proteins under different concentrations of urea and different pH.

Packaging

5 kg in poly drum

100, 500 g in poly bottle

1 kg in poly bottle