Not available outside of the UK & Ireland.

Application

Ellman′s reagent maybe used for acetylcholinesterase assay, and to specifically label cysteine residues. It is a sensitive reagent for measuring the free sulfhydryl content in proteins, peptides, and tissues.

General description

5,5′-Dithiobis(2-nitrobenzoic acid) is also called DTNB or Ellman′s reagent. It is a water soluble reagent, which interacts with thiol groups, and can be used conveniently at neutral pH. Thiolate anion (RS^-) reacts with Ellman′s reagent (DTNB^2-), to produce 2-nitro-5-thiobenzoate anion (TNB^2-) and one mixed disulfide (R-S-TNB^-). TNB^2- has a high molar absorption coefficient, and absorbs at 412nm. This absorption is independent of pH, at pH> 7.3.

Packaging

500 mg in glass insert

1, 5, 10, 25, 100 g in poly bottle