Triton X-100 solution, BioUltra, for molecular biology, approx. 10% in H2O

Code: 93443-100ml D2-231

Not available outside of the UK & Ireland.

Application

Triton-X has been used:for permeabilization of cells before techniques like indirect immunofluorescence and flow cytometryAs a component of lysis buffer used for ...


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£93.70 100ML
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Not available outside of the UK & Ireland.

Application

Triton-X has been used:for permeabilization of cells before techniques like indirect immunofluorescence and flow cytometryAs a component of lysis buffer used for homogenization of cellsAs a component of blocking buffer in immunofluorescence stainingFor the preparation of cells in double fluorescence staining

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

General description

Triton™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

absorptioncut-off at 310 nm
aggregation number100-155
cation tracesCd: ≤1 mg/kg, K: ≤100 mg/kg, Fe: ≤1 mg/kg, Mg: ≤1 mg/kg, Bi: ≤1 mg/kg, Mo: ≤1 mg/kg, Al: ≤1 mg/kg, Na: ≤100 mg/kg, Ni: ≤1 mg/kg, Cr: ≤1 mg/kg, Ca: ≤5 mg/kg, Zn: ≤1 mg/kg, Pb: ≤1 mg/kg, Co: ≤1 mg/kg, Ba: ≤1 mg/kg, Cu: ≤1 mg/kg, Mn: ≤1 mg/kg, Sr: ≤1 mg/kg, Li: ≤1 mg/kg
CMC0.2-0.9 mM (20-25°C), 0.23 mM
concentration~10% in H2O
density1.01 g/mL at 20 °C
descriptionnon-ionic
formliquid
gradefor molecular biology
HLB13.5
impuritiesphosphatases, none detected, RNases, none detected, proteases, none detected, insoluble matter, passes filter test, DNases, none detected
InChI keyHNLXNOZHXNSSPN-UHFFFAOYSA-N
InChI1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3
mol wtmicellar avg mol wt 80,000, average mol wt 625
pH6.5-8.5 (25 °C)
product lineBioUltra
Quality Level200
refractive indexn20/D 1.349±0.002
SMILES stringCC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1
transition tempcloud point 65 °C, pour point ~7 °C
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Description
Unit Size
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93443-500ML
Triton X-100 solution, BioUltra, for molecular biology, approx. 10% in H2O

Unit:500ML
List Price: £370.00
Source:List Price
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Cas Number9002-93-1
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