Phthaldialdehyde, for fluorescence, >=99.0% (HPLC)

Code: 79760-50g D2-231

Not available outside of the UK & Ireland.

Application

Phthaldialdehyde or OPA determines amino acids in proteins from animal tissue and food samples. Quantification of amino acids adducts of tyramine and phenylethyla...


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£508.00 50G

Not available outside of the UK & Ireland.

Application

Phthaldialdehyde or OPA determines amino acids in proteins from animal tissue and food samples. Quantification of amino acids adducts of tyramine and phenylethylamine produced by lactic acid bacteria used OPA as the pre-column derivative reagent for HPLC. Intracellular forms of glutathione (GSH/GSSG) are investigated using o-Phthalaldehyde as the pre-column fluorescent agent.

Biochem/physiol Actions

Phthaldialdehyde can be used for the pre-column derivatization of amino acids for high-performance liquid chromatography (HPLC) separation. It may also be used for flow cytometric measurements of protein thiol groups.

General description

Phthaldialdehyde, also known as o-Phthalaldehyde or OPA, is a pre-column (HPLC) derivatization reagent. OPA reacts with primary amino acids (AA) except cystine to form highly fluorescent adducts. The fluorescence of the derivatives is monitored at excitation and emission wavelengths of 345nm and 455nm, respectively. The HPLC technique efficiently separates the OPA-AA derivatives.

assay≥99.0% (HPLC)
fluorescenceλex 334 nm; λem 455 nm (Thiol Adduct), λex 340 nm; λem 450 nm in reaction buffer (with glycine)
formsolid
gradefor fluorescence
impurities≤0.5% phthalic acid
InChI keyZWLUXSQADUDCSB-UHFFFAOYSA-N
InChI1S/C8H6O2/c9-5-7-3-1-2-4-8(7)6-10/h1-6H
mp54-56 °C
Quality Level100
SMILES stringO=Cc1ccccc1C=O
storage temp.2-8°C
Code
Description
Unit Size
List Price
Qty
79760-5G
Unit:5G
List Price: £188.00
Source:List Price
ADD
79760-1G
Unit:1G
List Price: £57.20
Source:List Price
ADD
Cas Number643-79-8
Hazard Class8
Un Number2923
Pack GroupII
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