MES hydrate, BioUltra, >=99.5% (T)

Code: 69890-250g D2-231

Not available outside of the UK & Ireland.

General description

MES is one of the "Good" buffers developed for biological applications. MES is not recommended at pH 7.4; other buffers should be considered. All qualitie...


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£447.00 250G
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Not available outside of the UK & Ireland.

General description

MES is one of the "Good" buffers developed for biological applications. MES is not recommended at pH 7.4; other buffers should be considered. All qualities are tested for trace ions and all can be used for biological and biochemistry applications. This product is used in biochemical and biological techniques where highly purified products are needed.

Other Notes

Biological buffer; useful pH-range 5.8-6.5; Mobile phase component in brain tubulin isolation by amino-activated gel chromatography; Buffer in plant tissue culture.

Solubility/Stability: MES is soluble in water, giving a clear colourless solution at concentrations of 0.5M or higher. The pH of a solution should be between 2.5 and 5, depending on the concentration. Solutions are stable at 2-8°C for months. Sterilization: Sterilization should be by filteration through 0.2µM filters. Autoclaving is not recommended by any sulfonic acid buffers. If buffers must be nuclease-free, it is best to treat the water, then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably. The identity of the yellow breakdown product is unknown.

Easily compare specifications for MES hydrate products with the MES hydrate specification table.

Preparation Note

A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa=±1). Alternatively, volumes of equimolar MES free acid and sodium MES can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published.

λ0.5 M in H2O
anion tracessulfate (SO42-): ≤100 mg/kg, chloride (Cl-): ≤50 mg/kg
assay≥99.5% (T)
cation tracesBi: ≤5 mg/kg, Na: ≤50 mg/kg, Mo: ≤5 mg/kg, Al: ≤5 mg/kg, K: ≤50 mg/kg, As: ≤0.1 mg/kg, Li: ≤5 mg/kg, Ca: ≤20 mg/kg, Cr: ≤5 mg/kg, Mn: ≤5 mg/kg, Sr: ≤5 mg/kg, Mg: ≤5 mg/kg, Pb: ≤5 mg/kg, Cu: ≤5 mg/kg, Ba: ≤5 mg/kg, Fe: ≤5 mg/kg, Zn: ≤5 mg/kg, Ni: ≤5 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg
formpowder or crystals
ign. residue (900 °C)≤0.05%
impuritiesinsoluble matter, passes filter test
InChI keyMIIIXQJBDGSIKL-UHFFFAOYSA-N
InChI1S/C6H13NO4S.H2O/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);1H2
pH2.5-4.0 (25 °C, 0.5 M in H2O)
pKa 6.1
product lineBioUltra
Quality Level200
SMILES stringO.OS(=O)(=O)CCN1CCOCC1
solubilityH2O: 0.5 M at 20 °C, clear, colorless
useful pH range5.5-6.7
UV absorptionλ: 260 nm Amax: 0.025, λ: 280 nm Amax: 0.020
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Description
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69890-10G
MES hydrate, BioUltra, >=99.5% (T)

Unit:10G
List Price: £36.50
Source:List Price
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69890-50G
MES hydrate, BioUltra, >=99.5% (T)

Unit:50G
List Price: £134.00
Source:List Price
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Cas Number1266615-59-1
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