MES monohydrate, BioUltra, for molecular biology, >=99.5% (T)

Code: 69889-10g D2-231

Not available outside of the UK & Ireland.

Application

MES Monohydrate has been used:In the preparation of a solution used for the suspension and dilution of protoplasts to measure the density and number of protoplast...


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Not available outside of the UK & Ireland.

Application

MES Monohydrate has been used:In the preparation of a solution used for the suspension and dilution of protoplasts to measure the density and number of protoplasts using a haemocytometerAs a component of Murashige and Skoog basal salt mediaIn the preparation of total ionic strength adjustment buffer (TISAB)

General description

MES is one of the "Good" buffers developed for biological applications. MES is not recommended at pH 7.4; other buffers should be considered. All qualities are tested for trace ions and all can be used for biological and biochemistry applications. This product is used in molecular biology.

Other Notes

Solubility/Stability: MES is soluble in water, giving a clear colourless solution at concentrations of 0.5 M or higher. The pH of a solution should be between 2.5 and 5, depending on the concentration. Solutions are stable at 2-8C for months.Sterilization: Sterilization should be by filteration through 0.2 uM filters. Autoclaving is not recommended by any sulfonic acid buffers. If buffers must be nuclease-free, it is best to treat the water, and then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably. The identity of the yellow breakdown product is unknown.

Preparation Note

A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa =±1). Alternatively, volumes of equimolar MES free acid and sodium MES can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published.

λ0.5 M in H2O
anion tracessulfate (SO42-): ≤50 mg/kg, chloride (Cl-): ≤50 mg/kg
assay≥99.5% (T)
cation tracesBi: ≤5 mg/kg, Na: ≤50 mg/kg, Mo: ≤5 mg/kg, Al: ≤5 mg/kg, K: ≤50 mg/kg, As: ≤0.1 mg/kg, Li: ≤5 mg/kg, Ca: ≤20 mg/kg, Cr: ≤5 mg/kg, Mn: ≤5 mg/kg, Sr: ≤5 mg/kg, Mg: ≤5 mg/kg, Pb: ≤5 mg/kg, Cu: ≤5 mg/kg, Ba: ≤5 mg/kg, Fe: ≤5 mg/kg, Zn: ≤5 mg/kg, Ni: ≤5 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg
formpowder or crystals
gradefor molecular biology
ign. residue (900 °C)≤0.05% (as SO4)
impuritiesInsoluble matter, passes filter test, RNases, none detected, Proteases, none detected, DNases, none detected, Phosphatases, none detected
InChI keyMIIIXQJBDGSIKL-UHFFFAOYSA-N
InChI1S/C6H13NO4S.H2O/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);1H2
mp>300 °C (lit.)
pH2.5-4.0 (25 °C, 0.5 M in H2O)
pKa (25 °C)6.1
product lineBioUltra
Quality Level200
SMILES stringO.OS(=O)(=O)CCN1CCOCC1
solubilityH2O: 0.5 M at 20 °C, clear, colorless
useful pH range5.5-6.7
UV absorptionλ: 260 nm Amax: 0.025, λ: 280 nm Amax: 0.020
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69889-250G
MES monohydrate, BioUltra, for molecular biology, >=99.5% (T)

Unit:250G
List Price: $546.65
Source:List Price
ADD
69889-50G
MES monohydrate, BioUltra, for molecular biology, >=99.5% (T)

Unit:50G
List Price: $168.20
Source:List Price
ADD
Cas Number145224-94-8
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