The cell culture flask CELLine is a disposable bioreactor based on two-compartment technology. It allows to cultivate cells at highest densities (10^7-10^8 cells/ml) and to express proteins like monoclonal antibodies at very high concentrations.
The bioreactor CELLine is separated into a medium and a cell compartment by means of a 10 kDa semi-permeable membrane. This membrane allows a continuous diffusion of nutrients into the cell compartment with a concurrent removal of any inhibitory waste. The individual accessibility of the compartments allows supplying cells with fresh medium without mechanically interfering with the culture. Optimal oxygen supply and control of carbon dioxide levels is ensured by a silicone membrane which forms the cell compartment base.
CELLine classic (CL) is ideal for laboratory scale applications using suspension cells or adherent cells in combination with microcarriers. The unit is optimised for cultivation of hybridomas and many other cell types (e.g. CHO, NSO, SF cells).
CELLine adhere (AD) is specifically adapted to allow growth of anchorage-dependent cells (e.g. HEK, BHK, CHO cells). The bioreactor contains a woven, polyethylene terephtalate (PET) matrix in the cell compartment providing an ideal surface for cell attachement.
This product has met the following criteria: