Cole-Parmer SP-400-BIO UV/Visible Diode Array Scanning Spectrophotometer

Code: spe4506 E37-31

This simple, low cost, easy to use UV/visible spectrophotometer is dedicated for life science applications. The fast, responsive color touchscreen and pre-programmed methods for the measurement of ...


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$4,490.65 EACH

2 Year Warranty
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This simple, low cost, easy to use UV/visible spectrophotometer is dedicated for life science applications. The fast, responsive color touchscreen and pre-programmed methods for the measurement of nucleic acid concentration and purity, protein assays and cell density, makes this spectrophotometer the ideal addition to any laboratory. The spectrophotometer utilizes diode array technology to scan the entire wavelength range (198 to 800nm) in less than 3 seconds and the narrow spectral bandwidth of 3nm, makes it ideal to identify peaks for determining nucleic acid purity. The 1024 element diode array detector coupled with a flash xenon lamp results in a long life, robust spectrophotometer.

The Nucleic Acids measurement mode can be used to quantify the concentration and purity of dsDNA, ssDNA, RNA and oligonucleotides; using wavelengths recorded at 260, 280 and 230nm, with an optional correction at 320nm. The concentration is calculated along with the corresponding ratios. At the touch of a button it is easy to check the purity of the nucleic acids. This is done by identifying distorted peaks in the purity scan between 200 and 350nm. This is especially useful for RNA samples where impurities may be present at 230nm, but cannot be detected using the 260/280nm ratio measurement.

The Proteins measurement mode can be used to calculate protein concentration by creating standard curves from protein assay kits. With pre-programmed methods for measuring Bradford, Lowry, Biuret and Bicinchoninic Acid (BCA) assays; up to 6 standards can be measured with 3 replicates of each standard to minimise any dilution errors. The Proteins measurement mode is also pre-programmed with the Direct UV and Warburg-Christian methods to determine protein purity.

The spectrophotometer has a pre-programmed method for measuring optical density of bacterial cultures such as E.Coli and yeast cells. This is ideal to measure cell growth before cell harvesting. As well as these pre-programmed methods this versatile spectrophotometer has measurement modes for simple photometrics, concentration, quantitation, spectrum scanning and kinetics. Enabling measurements to be performed at any selected wavelength between 198 to 800nm.

Use the preprogrammed nucleic acid and protein measurement modes to calculate concentration levels. The nucleic acids measurement mode quantifies the concentration and purity of dsDNA, sDNA, RNA, and oligonucleotides. A simple touch of a button can check the purity of the nucleic acids. Calculate the protein concentration using the protein measurement mode. Preprogrammed methods for measuring Bradford, Lowry, Biuret, and Bicinchoninic Acid (BCA) assays minimise errors.

  • Pre-programmed for DNA, RNA and protein analysis
  • Compatible with ultra-micro, semi-micro, micro and macro cuvettes
  • Scanning diode array technology
  • Colour touchscreen navigation Small footprint and lightweight (3kg)
  • Fast scan speed
  • English, French, German, Italian and Spanish language options
  • Multiple USB ports for data storage and printer connectivity
  • Dedicated to life science applications
  • Narrow spectral bandwidth of 3nm identifies peaks for determining nucleic acid purity
  • Wavelength range: 198 to 800nm
  • Spectral bandwidth is 3nm

Specifications
Wavelength Range, nm198 to 800
Wavelength Accuracy, nm±2
Wavelength Repeatability, nm±2
Spectral Bandwidth, nm3
Absorbance Range, A-0.300 to 2.500
Absorbance Accuracy, A±0.01 (at 1.0A and 546nm)
Absorbance Stability, A/h±0.005 (at 0.04A and 546nm)
Noise, A±0.002 (at 0.04A), ±0.02A (at 2.0A and 546nm)
Stray Light at 340nm, %T< 1%T according to ANSI/ASTM E387- 72
Dims, w x d x h, mm212 x 422 x 120
Weight, kg2.8
Code
Description
Unit Size
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SPE2234
Unit:EACH
List Price: $642.35
Source:List Price
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SPE3514
Unit:EACH
List Price: $204.45
Source:List Price
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SPE4510
Unit:EACH
List Price: $204.45
Source:List Price
ADD
Absorbance Range, A-0.300 to 2.500
Dims, w x d x h, mm212 x 422 x 120
Wavelength Accuracy, nm±2nm
Weight, kg2.8
Wavelength Range, nm198 - 800
This product has met the following criteria to qualify for the following awards:


Sustainable Distribution & Packaging
  • Manufactured in the UK or Ireland
  • Country of origin has an Environmental Performance Index (EPI) greater than 60

To find out more about our Sustainability Attributes, click here.

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