Bleomycin sulfate from Streptomyces verticillus, crystalline, 1.5-2.0 units/mg solid

Code: b5507-15un D2-231

Not available outside of the UK & Ireland.

Application

Bleomycin Sulfate is an atineoplastic antibiotic isolated from Streptomyces verticillus. It is recommended for use as a cell culture agent at 10-100 ...


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Not available outside of the UK & Ireland.

Application

Bleomycin Sulfate is an atineoplastic antibiotic isolated from Streptomyces verticillus. It is recommended for use as a cell culture agent at 10-100 µg/mL.

Bleomycin sulfate from Streptomyces verticillus has been used: to study the impact of telomere shortening in lung fibrosisto induce lung injury and study its effect on CEACAM6 expressionas a DNA-alkylating agent to induce lung injurystudy its effect on CEACAM6 expression to cause lung injurysubsequent fibrosis in animals

Biochem/physiol Actions

Bleomycin sulfate binds to DNA, causes ssDNA scission at specific base sequences and inhibits DNA synthesis. This inhibitory action requires bleomycin to bind oxygen and a metal ion. It can also cleave RNA, to a lesser degree but more selectively. It acts as an inducer and regulator of apoptosis and inhibits tumor angiogenesis.

Caution

This product should be stored at 2-8°C.

Components

This product is a mixture of glycopeptides antibiotics containing Bleomycin A2 (70%) and B2(30%). Bleomycins differ from each other in the terminal amine and show varying biological activity.

Features and Benefits

This compound is a featured product for Apoptosis research. Click here to discover more featured Apoptosis products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

General description

Bleomycin Sulfate is an antineoplastic antibiotic isolated fromStreptomyces verticillus.It is recommended for use as a cell culture agent at 10-100 μg/mL.

Chemical structure: glycopeptide

Other Notes

Keep container tightly closed in a dry and well-ventilated place, strongly hygroscopic

Packaging

15 units in serum bottle

Preparation Note

The product is soluble in water in water at 20 mg/mL and is active for several days. A solution of 2 units/mL in 0.1 M neutral potassium phosphate should be used within 14 days. A solution is active for three months at 3 units/mL in normal saline.

antibiotic activity spectrumfungi
cation tracesCu: ≤0.10%
colorwhite
formcrystalline
InChI keyOOXTWFJZZAJGKA-CNLAFNBISA-N
InChI1S/2C55H83N17O21S3.H2O4S/c2*1-20-33(69-46(72-44(20)58)25(12-31(57)76)64-13-24(56)45(59)82)50(86)71-35(41(26-14-61-19-66-26)91-54-43(39(80)37(78)28(16-73)90-54)92-53-40(81)42(93-55(60)88)38(79)29(17-74)89-53)51(87)67-22(3)36(77)21(2)47(83)70-34(23(4)75)49(85)63-10-8-32-68-27(18-94-32)52-65-15-30(95-52)48(84)62-9-7-11-96(5)6;1-5(2,3)4/h2*14-15,18-19,21-25,28-29,34-43,53-54,64,73-75,77-81H,7-13,16-17,56H2,1-6H3,(H13-,57,58,59,60,61,62,63,66,67,69,70,71,72,76,82,83,84,85,86,87,88);(H2,1,2,3,4)/t2*21-,22+,23+,24-,25-,28-,29+,34+,35+,36-,37+,38+,39-,40-,41-,42+,43-,53-,54-;/m00./s1
mode of actionDNA synthesis | interferes
Quality Level200
SMILES string[O-]S([O-])(=O)=O.C[C@@H](O)[C@@H](NC(=O)[C@@H](C)[C@H](O)[C@@H](C)NC(=O)[C@H](NC(=O)c1nc(nc(N)c1C)[C@H](CC(N)=O)NC[C@H](N)C(N)=O)[C@@H](O[C@@H]2O[C@@H](CO)[C@@H](O)[C@H](O)[C@@H]2O[C@@H]3O[C@H](CO)[C@@H](O)[C@@H](OC(N)=O)[C@@H]3O)c4c[nH]cn4)C(=O)NCCc5nc(cs5)-c6ncc(s6)C(=O)NCCC[S+](C)C.C[C@@H](O)[C@@H](NC(=O)[C@@H](C)[C@H](O)[C@@H](C)NC(=O)[C@H](NC(=O)c7nc(nc(N)c7C)[C@H](CC(N)=O)NC[C@H](N)C(N)=O)[C@@H](O[C@@H]8O[C@@H](CO)[C@@H](O)[C@H](O)[C@@H]8O[C@@H]9O[C@H](CO)[C@@H](O)[C@@H](OC(N)=O)[C@@H]9O)cc[nH]cn)C(=O)NCCcnc(cs)-cncc(s)C(=O)NCCC[S+](C)C
solubilityH2O: 20 mg/mL
specific activity1.5-2.0 U/mg
storage temp.2-8°C
Cas Number9041-93-4
This product has met the following criteria: