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Analysis Note

ControlMouse N1E-115 neuroblastoma cells

Application

Immunohistochmeistry Analysis: A 1:50 dilution from a representative lot detected Alzheimer Precursor Protein A4 in adult mouse brain tissue.

Anti-Alzheimer Precursor Protein A4 Antibody, clone 22C11, Alexa488 Conj. is an antibody against Alzheimer Precursor Protein A4 for use in IHC, ICC.

General description

Deposits of amyloid protein in senile plaques near nerve processes are found in the brains of aged human and cases of Alzheimer′s Disease. The principle component of this extracellular amyloid is beta A4, a 4 kDa peptide derived from a larger amyloid precursor protein (APP), which is widely expressed in the brain and body. The creation, transport and function of these proteins is currently an active area of research.

Immunogen

Epitope: a.a. 66-81 of APP (N-terminus)

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Quality

Evaluated by Immunocytochemistry in mouse N1E-115 neuroblastoma cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Alzheimer Precursor Protein A4 in mouse N1E-115 neuroblastoma cells.

Specificity

Unconjugated MAB348 recognizes N-terminal amino acids 66-81 on the pre-A4 molecule (Hilbich et al., 1993). 22C11 recognizes all three isoforms of APP: immature, ~110kDa; sAPP, ~120kDa; and mature, ~130kDa (Hoffmann et al., 2000). This antibody is known to cross react with APLP2 (Slunt, 1994).

Target description

Reacts with pre-A4. The unconjugated antibody (MAB348) recognizes all three isoforms of APP, immature ~110kDa, sAPP ~120kDa, and mature ~130kDa (Hoffmann et al., 2000).