Not available outside of the UK & Ireland.

Analysis Note

ControlMouse brain, rat brain tissue.

Application

Immunocytochemistry:
Representative image from a previous lot.
Confocal fluorescent analysis of PC12 using MAb1596 Mouse mAB (Red). Actin filaments have been labeled with Alexa Fluor 488 -Phalloidin (Green). Nuclear is stained with DAPI (Blue). Membrane staining positive.

Immunocytochemistry:
12.5 µg/mL

Western Blot:
1-5 µg/mL (ECL) on rat brain lysate (20 µg).

Optimal working dilutions must be determined by the end user.

Research CategoryNeuroscience

Anti-Post Synaptic Density Protein 95 Antibody, clone 6G6-1C9 detects level of Post Synaptic Density Protein 95 & has been published & validated for use in IC, IH & WB.

Research Sub CategorySynapse & Synaptic Biology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

PSD-95 is part of a multiprotein complex restricted to the post-synaptic membrane. It is believed to initiate NMDA receptor clustering on the post synaptic membrane. Its restricted expression makes it an excellent marker for post-synaptic membrane regions on neurons.

Immunogen

Recombinant rat PSD-95.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-1066

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

See datasheet for purification method.

Format: Purified

Purified immunoglobulin. Liquid in PBS, pH 7.2 with 50% glycerol and 0.09% sodium azide.

Quality

Routinely evaluated in Immunocytochemistry.

Specificity

Other species have not been tested.

Reacts with a protein of ~100 kDa, corresponding to the apparent molecular weight of PSD-95, on SDS-PAGE immunoblots of samples from rat, mouse and bovine. A slightly higher molecular weight band is also detected. In rat brain and mouse brain tissue extracts a band of ~75 kDa is also present.

Storage and Stability

Stable for 6 months at -20°C in undiluted aliquots from date of receipt. Do not store below -20°C.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Target description

~100 kDa