Not available outside of the UK & Ireland.

Analysis Note

ControlMouse brain tissue, rat astrocytes, rat neurons.

Application

Anti-Glial Fibrillary Acidic Protein (GFAP) Antibody is an antibody against Glial Fibrillary Acidic Protein (GFAP) for use in IC, IH, IH(P) with more than 65 product citations.

Research Sub CategoryNeuronal & Glial Markers

Research CategoryNeuroscience

Immunohistochemistry(paraffin):
Representative images from a previous lot.

Immunocytochemistry:
A 1:1,000 dilution of a previous lot was used in Immunocytochemistry.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.

Immunogen

Purified bovine GFAP

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: AB1540

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Rabbit serum. Liquid. Contains no preservative.

Unpurified

Quality

Routinely evaluated by immunohistochemistry on brain tissue, astrocytes, and neurons.

Immunohistochemistry(paraffin) Analysis:
GFAP (IHC2078) staining of Human Brain. Tissue pretreated with Citrate Buffer, pH 6.0. Pre-diluted polyclonal antibody, using IHC-Select™ Detection with HRP-DAB. Glial cells stain strongly (brown).
Optimal Staining with Citrate Buffer Epitope Retrieval: Human Brain

Specificity

It is expected that the antibody will react with most mammalian GFAP due to homology. Reactivity with other species has not been confirmed.

Recognizes Glial Fibrillary Acidic Protein (GFAP).

Storage and Stability

Stable for 1 year at -20°C undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

50 kDa