Not available outside of the UK & Ireland.

Application

Western blot was performed to analyze purified proteins over expressed in E.coli using alkaline-phosphatase-conjugated goat anti-rat IgG at a 1:1000 dilution. Western blot was developed using 5-bromo-4-chloro-3indolyl phosphate/nitro blue tetrazolium tablets (Sigma).

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Western Blotting (1 paper)

Anti-rat IgG (whole molecule)–alkaline phosphatase antibody can be used in ELISA and western blot assays.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

IgG, a monomer, is the predominant antibody circulating in blood which assist in phagocytic destruction of foreign microorganism. Anti-rat IgG (whole molecule)-alkaline phosphatase antibody (diluted 1: 30 000) can be used in rat MPO (myeloperoxidase) ELISA. It may also use as secondary antibody in immunodetection method. Anti-rat IgG (whole molecule)-alkaline phosphatase antibody reacts specifically with all rat immunoglobulins.

Immunogen

Purified rat IgG.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl₂, 10 mM glycine, 50% glycerol and 15 mM sodium azide.