Not available outside of the UK & Ireland.

Application

ELISA (see application references)Immunoblotting (1:1000)Immunoprecipitation (see application references and comments)Immunofluorescence (see application references)

General description

This Anti-Ubiquitin Mouse mAb (FK2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation, Immunofluorescence for the detection of Ubiquitin.

Recognizes K²⁹-, K⁴⁸-, and K⁶³-linked polyubiquitinylated and monoubiquitinylated proteins, but not free ubiquitin, in Raji cells.

Mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with myeloma cells. Recognizes K²⁹-, K⁴⁸-, and K⁶³-linked polyubiquitinylated and monoubiquitinylated proteins.

Immunogen

human

unpurified polyubiquitinylated lysozyme

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Does not recognize free ubiquitin. For immunoprecipitation, once the antibody is immobilized, it demonstrates affinity for free ubiquitin as well as for poly- and monoubiquitinylated proteins. Antibody should be titrated for optimal results in individual systems.

Matsumoto, M., et al. 2005. Proteomics 5, 4545.Boname, J.N., et al. 2001. Immunity 15, 627.Campbell, V., et al. 2001. Neuron 32, 1013.Fujimuro, M., et al. 2003. FEBS Letts. 349, 173.

Packaging

100 µg in Glass bottle

Please refer to vial label for lot-specific concentration.

Physical form

In PBS.

Preparation Note

Raji cells

Reconstitution

Following initial thaw, aliquot and freeze (-20°C). Store diluted antibody at 4°C for up to 1 month.

Warning

Toxicity: Standard Handling (A)