Not available outside of the UK & Ireland.

Application

Anti-Muc4 Antibody, clone 8G7 is a highly specific mouse monoclonal antibody, that targets Mucin & has been tested in western blotting & IHC.

Research CategoryCell Structure

Research Sub CategoryECM Proteins

Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Muc4 in human ilium tissues.

Western Blotting Analysis: A representative lot from an independent laboratory detected Muc4 in maligant melanoma and cutaneous squamous cell carcinoma tissue lysates (Moniaux, N., et al. (2004). J Histochem Cytochem. 52(2):253-261.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Muc4 in lung adenocarcinoma and pancreatic adenocarcinoma tissues (Moniaux, N., et al. (2004). J Histochem Cytochem. 52(2):253-261.).

Immunohistochemistry Analysis: A representative lot from independent laboratories detected Muc4 in 330 tissue spots representing normal skin, and benign and malignant cutaneous diseases (Singh, A. P., et al. (2006). Prostate. 66(4):421-429.; Chakraborty, S., et al. (2010). J Clin Pathol. 63(7):579-584.).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Mucin-4 (MUC-4 or Muc4) is also called Ascites sialoglycoprotein (ASGP), Pancreatic adenocarcinoma mucin, Testis mucin, and Tracheobronchial mucin. Muc4 is proteolytically cleaved into two chains: mucin-4 alpha chain and mucin-4 beta chain. Muc4 has anti-adhesive properties and is involved in tumor progression, cell proliferation and differentiation of epithelial cells. Muc4 is expressed in the thymus, thyroid, lung, trachea, esophagus, stomach, small intestine, colon, testis, prostate, ovary, uterus, placenta, mammary and salivary glands as well as lung cancers, squamous cell carcinomas of the upper aerodigestive tract, mammary carcinomas, biliary tract, colon, cervix cancers, pancreatic tumors, and pancreatic tumor cell lines. The expression of Muc4 has become a very useful indicator of poor prognosis in patients with invasive ductal carcinoma and intrahepatic cholangiocarcinoma, mass forming type (IDC,ICC-MF), where patients with high Muc4 expression had lower survival rates than those with low Muc4 expression.

Immunogen

KLH-conjugated linear peptide corresponding to the beta chain tandem repeat region of human Muc4.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl without preservatives.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in CD18/HPAF cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Muc4 in 10 µg of CD18/HPAF cell lysate.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~240 kDa observed. Uniprot describes a molecular weight at 430 kDa; however, this protein may be observed at ~240 kDa due to alternative spicing and post-translational modification (Moniaux, N., et al. (2004). J Histochem Cytochem. 52(2):253-261.).