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Analysis Note

ControlVesicular Acetylcholine Transporter (VAChT) peptide

Application

Research Sub CategorySignaling Neuroscience

Research CategoryNeuroscience

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Vesicular Acetylcholine Transporter (VAChT) in rat midbrain tissue.
ELISA Analysis: A representative lot detected Vesicular Acetylcholine Transporter (VAChT) in a direct ELISA using a corresponding peptide.

Anti-Vesicular Acetylcholine Transporter (VAChT) Antibody detects level of Vesicular Acetylcholine Transporter (VAChT) & has been published & validated for use in IHC, ELISA.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Acetylcholine mediates cholinergic transmission in the central and peripheral nervous systems, and is an important modulator of cognition, movement, and cardiovascular function, among other processes. The effects of acetylcholine are regulated by its synthesis, storage, and breakdown, which involve the related enzymes choline acetyltransferase (ChAT), vesicular acetylcholine transporter (VAChT), and acetylcholinesterase (AChE), respectively. VAChT is primarily found in the membrane of presynaptic secretory vesicles. It contains 12 transmembrane domains and a synaptic-vesicular–targeting signal located on the intracellular C-terminal tail. The primary function of VAChT is to actively transport cytoplasmic ACh, across an electrochemical gradient, into synaptic vesicles that release ACh upon cell stimulation. The VAChT can affect cholinergic signaling by affecting the relative pools of ACh secreted to activate postsynaptic nicotinic and muscarinic receptors. VAChT is phosphorylated on its serine 480 residue in the C-terminal tail by protein kinase C. Although the precise role of this modification is still unclear, previous studies suggest that it may affect the localization of VAChT to synaptic vesicles.

Immunogen

KLH-conjugated linear peptide corresponding to the C-terminus of rat VAChT.

Epitope: C-terminus

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified goat polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Immunohistochemistry Analysis.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Vesicular Acetylcholine Transporter (VAChT) in mouse midbrain tissue.

Specificity

Other homologies: Human (95% sequence homology).

This antibody recognizes the C-terminus of Vesicular Acetylcholine Transporter (VAChT).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

57 kDa calculated