Not available outside of the UK & Ireland.

Analysis Note

ControlPositive Control: Kidney, muscle, tendon spleen tissue
Negative Control: Neurons/glia

Application

Detect Collagen Type IV using this Anti-Collagen Type IV Antibody validated for use in DB, ELISA, IC, IH.

Research CategoryCell Structure

Research Sub CategoryECM Proteins

Dot and slot blotting: 1:100-1:500

ELISA: 1:1000-1:4000

Indirect immunohistochemistry (frozen sections only): 1:10-1:40

Immunocytochemistry 1:10-1:20

Optimal working dilutions must be determined by the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Human and bovine placental collagen type IV

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Sepharose is a registered trademark of Cytiva

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

ImmunoAffinity Purified

Affinity Purified immunoglobulin. Prior to purification the antisera was adsorbed against collagen type I, II, III, V and VI immobilized on Sepharose^® 4B. Liquid in borate buffered saline, pH 8.2, no sodium azide.

Specificity

Recognizes Human and bovine type IV collagen as demonstrated by ELISA. Less than 10% cross reactivity with collagen types I, II, III, V, and VI. May show reactivity to type IV collagen from other species. AB769 has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin).

Storage and Stability

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.