Not available outside of the UK & Ireland.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Western Blotting (1 paper)

Anti-AtMPK6 antibody produced in rabbit has been used in immunoblotting.

Biochem/physiol Actions

Mitogen-activated protein kinase (MAPK) cascades are among the most conserved signal transduction pathways in eukaryotes. MAPKs regulate a variety of cellular activities ranging from gene expression, cell proliferation, motility, metabolism and apoptosis. In Arabidopsis thaliana putative functions have been assigned to AtMPK3, AtMPK4 and AtMPK6 and all three MAPKs have been linked to diverse stress responses, including oxidative stress signaling, pathogen defense, drought, high salt, cold and touch.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

In Arabidopsis thaliana, 20 genes (MPKs) encode putative plant MAPKs, which can be grouped into four or five classes according to sequence similarity. All plant MAPKs are classified into the plant extracellular-regulated kinase/ERK (PERK) subfamily.

Immunogen

synthetic peptide corresponding to the C-terminus of Arabidopsis thaliana MPK6 (amino acids 384-395). This sequence is specific to AtMPK6 (not found in the other 20 known AtMPK isoforms). It shares limited homology (40-50% identity) to the AtMPK3 and AtMPK4 isoforms, and is highly conserved in the Nicotiana tabacum ortholog SIPK.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.