Not available outside of the UK & Ireland.

Application

Dot Blot (1:1000)r>Immunoblotting (1:1000)r>Immunofluorescence (1:1000)r>Frozen Sections (1:1000; see application references)

General description

This Anti-Tyrosine Hydroxylase Rabbit pAb is validated for use in Dot Blot, Immunoblotting, Immunofluorescence, Frozen Sections for the detection of Tyrosine Hydroxylase.

Recognizes the ~60 kDa tyrosine hydroxylase protein in okadaic acid-treated PC12 cells.

Affinity purified rabbit polyclonal antibody. Recognizes the ~60 kDa tyrosine hydroxylase protein.

Immunogen

Rat

purified, SDS-denatured rat pheochromocytoma tyrosine hydroxylase

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Cross-reacts with most mammalian forms and some non-mammalian forms of tyrosine hydroxylase. Variables associated with assay conditions will dictate the proper working dilution.

Xu, Z.Q. 1998. Neurosci.82, 727.Haycock, J.W. 1993. Anal. Biochem.208, 397.Lewis, D.A., et al. 1993. Neuroscience54, 477.Haycock, J. 1989. Anal. Biochem.181, 259.

Packaging

100 µL in Plastic ampoule

Physical form

In 150 mM NaCl, 10 mM HEPES, 50% glycerol, 0.01% BSA, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Warning

Toxicity: Standard Handling (A)