Not available outside of the UK & Ireland.

Analysis Note

Complete activation from homogeneous prothrombin by SDS-PAGE

Application

Thrombin, bovine, high activity has been used: to stimulate LNCaP cell and to study the effect of activation of G-protein-coupled receptors (GPCRs) in fibrin gel fabricationto form fibrinogen gel by fibrinogen gel-based method

Biochem/physiol Actions

Thrombinconverts fibrinogen into fibrin and activates factors V, VIII, XI, and XIII. It promotes platelet activation and vascular contraction. Thrombinstabilizes the fibrin polymers. It plays a crucial role in the final stages of the blood coagulation cascade.

General description

Thrombin is a sodium-activated type II enzyme. This serine protease enzyme consists of two anion bindingexosites, ABE-I and ABE-II.Thrombin is synthesized from zymogen prothrombin (factor II) in theliver.BovineA-thrombin comprises a light chain (A chain)and a heavy chain (B chain). These two chains are linked by non-covalent interactions and by one disulfidebond.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

100, 500 u in Plastic ampoule

Physical form

Lyophilized from 200 mM NaCl, 50 mM sodium citrate, 1 ml 0.1% PEG, pH 6.5.

Warning

Toxicity: Harmful (C)