Not available outside of the UK & Ireland.

Application

Enzyme Immunoassay (10 µg/ml, see application references)

General description

This Mouse Anti-Human IgG₄, Fc Fragment Specific (HP6025) is validated for use in Enzyme Immunoassay for the detection of Human IgG₄, Fc Fragment Specific.

Purified mouse monoclonal antibody. Recognizes human IgG₄ Fc fragment.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Antibody should be titrated for optimal results in individual systems.r>r>Cross-reactivity by ELISA against human myeloma proteins:r>Human IgG₁: ≤0.1%r>Human IgG₂: ≤0.04%r>Human IgG₃: ≤0.02%r>Human IgG₄: 100%r>Human IgA: ﹤0.1%r>Human IgE: ﹤0.1%r>Human IgM: ﹤0.1%r>r>Cross-reactivity by ELISA against chimeric antibodies:r>Human IgG₁: ﹤0.01%r>Human IgG₂: ﹤0.01%r>Human IgG₃: ﹤0.01%r>Human IgG₄: 100%r>Human IgA: ﹤0.01%r>Human IgE: ﹤0.01%r>Human IgM: ﹤0.01%

Hamilton, R.G. 1990. Ann. Biol. Clin.48, 473.Fasullo, F.J., et al. 1989. Clin. Chem.35, 364.Jefferis, R., et al. 1986. Immunol. Letters10, 223.

Packaging

400 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C). Avoid more than 5 freeze/thaw cycles.

Warning

Toxicity: Standard Handling (A)