Not available outside of the UK & Ireland.

Application

ELISA (1:500-1:2000, see comments)

General description

This Mouse Anti-Human IgG₃, Hinge Specific (HP6047) Biotin Conjugate is validated for use in ELISA for the detection of Human IgG₃, Hinge Specific.

Purified mouse monoclonal antibody conjugated to biotin. Recognizes human IgG₃ hinge region.

Immunogen

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Biotinylated with hydroxysuccinimide ester biotin. This biotin-labeled antibody is an extremely versatile reagent because of the high binding affinity for avidin and avidin-labeled compounds. Variables associated with assay conditions will dictate the proper working dilution.r>r>Cross-reactivity by ELISA against human myeloma proteins:r>Human IgG₁: ≤0.1%r>Human IgG₂: ≤1%r>Human IgG₃: 100%r>Human IgG₄: ≤0.1%r>Human IgA: ≤0.1%r>Human IgE: ≤0.1%r>Human IgM: ≤0.1%r>r>Cross-reactivity by ELISA against chimeric antibodies:r>Human IgG₁: ﹤0.001%r>Human IgG₂: ﹤0.001%r>Human IgG₃: 100%r>Human IgG₄: ﹤0.001%r>Human IgA: ﹤0.001%r>Human IgE: ﹤0.001%r>Human IgM: ﹤0.001%

Hamilton, R.G. 1990. Ann. Biol. Clin.48, 473.Fasullo, F.J., et al. 1989. Clin. Chem.35, 364.Hamilton, R.G. 1987. Clin. Chem.33, 1707.Jefferis, R., et al. 1986. Immunol. Letters10, 223.

Packaging

200 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, 1% BSA, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)