Not available outside of the UK & Ireland.

Biochem/physiol Actions

ED₅₀ = 50-200 pg/ml as measured by its ability to stimulate the proliferation of PHA-activated human lymphoblasts

General description

Recombinant, human interleukin-12 expressed in S. frugiperda insect cells. Pleiotropic cytokine that is produced by monocytes/macrophages, B-cells, and connective tissue type mast cells. Also known as natural killer cell stimulatory factor and cytotoxic lymphocyte maturation factor. A potent inducer of IFN-γ production and T-cell differentiation and function.

Recombinant, human interleukin-12 expressed in S. frugiperda insect cells. Pleiotropic cytokine that is produced by monocytes/macrophages, B cells, and connective tissue-type mast cells. Also known as natural killer cell stimulatory factor and cytotoxic lymphocyte maturation factor. A potent inducer of IFN-γ production and T cell differentiation and function.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Kang, K., et al. 1996. J. Immunol. 156, 1402.Riemann, H., et al. 1996. J. Immunol. 156, 1799.Brunda, M.J., et al. 1994. J. Leukoc. Biol. 55, 280.Smith, T.J., et al. 1994. Eur. J. Immunol. 24, 822.Gubler, U., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 4143.Stern, A.S., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 6808.Kobayashi, M., et al. 1989. J. Exp. Med. 170, 827.

Packaging

5 µg in Plastic ampoule

Physical form

Lyophilized from sterile-filtered PBS, 50 µg BSA/µg IL-12.

Reconstitution

Reconstitute to a concentration ≥1 µg/ml with sterile PBS containing at least 0.1% HSA or BSA.

Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 1 month at 4°C. Avoid freeze/thaw cycles of solutions.

Warning

Toxicity: Standard Handling (A)