Not available outside of the UK & Ireland.

Biochem/physiol Actions

ED₅₀ = 0.2-1 ng/ml as measured in a cell proliferation assay using a mouse mast cell line, MC/9.

General description

Recombinant, human interleukin-10 expressed in S. frugiperda insect cells. Pleiotropic cytokine that can exert either immunosuppressive or immunostimulatory effects on a variety of cell types. Potent modulator of monocyte/macrophage function. Exhibits anti-inflammatory effects by suppressing macrophage proliferation. Human IL-10 has cross-species reactivity and is active in mouse cell lines.

Recombinant, human interleukin-10 expressed in S. frugiperda insect cells. Pleiotropic cytokine that can exert either immunosuppressive or immunostimulatory effects on a variety of cell types. Potent modulator of monocyte/macrophage function. Exhibits anti-inflammatory effects by suppressing macrophage proliferation. Blocks lipopolysaccharide induction of MMP-2 and MMP-9. Known to reduce neuronal degeneration following CNS injury by blocking glutamate-induced caspase-3 activation. Human IL-10 is also active on murine cell lines.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Bachis, A., et al. 2001. J. Neurosci.21, 3104.Fortunato, S.J., et al. 2001. Obstet. Gynecol.98, 284.Fleming, S.D., et al. 1996. J. Immunol. 156, 1143.Wang, C.Q., et al. 1996. J. Cell Physiol. 166, 305.Niro, H., et al. 1994. Int. Immunol. 6, 661.Moore, K., et al. 1993. Annu. Rev. Immunol. 11, 165.Vieira, P., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 1172.

Packaging

5 µg in Plastic ampoule

Physical form

Carrier-free.

Reconstitution

Reconstitute in sterile water or buffer to a final concentration of 0.1-1 mg/ml as stock solution. Further dilute in buffer such as PBS, 1% BSA.

Following reconstitution, aliquot and freeze -70°C. Stock solutions are stable for up to 3 months at -70°C. Avoid freeze/thaw cycles of solutions.

Warning

Toxicity: Standard Handling (A)